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fluorescence activated cell sorting facs assays calcein am  (MedChemExpress)


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    Structured Review

    MedChemExpress fluorescence activated cell sorting facs assays calcein am
    Fluorescence Activated Cell Sorting Facs Assays Calcein Am, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 96/100, based on 110 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/calcein+am/Calcein-AM/pm42298670-157-0-8
    Average 96 stars, based on 110 article reviews
    fluorescence activated cell sorting facs assays calcein am - by Bioz Stars, 2026-10
    96/100 stars

    Images

    Related Articles

    FACS:

    Article Title: Astrocytic Mitochondria Transplantation Rescues Neuron Loss and Dendritic Injuries in Acute Cerebral Ischemic Stroke Mouse Model by Flexibly Regulating Mitochondria Dynamics
    Article Snippet: .. HT‐22 cells were prepared in 4 tubes for each FACS run: experimental group stained with both Calcein‐AM (2μM) and PI (1.5μM) (HY‐K1073, MCE, United States), single‐color controls for each fluorophore, and unstained cells for background determination. ..

    Staining:

    Article Title: Astrocytic Mitochondria Transplantation Rescues Neuron Loss and Dendritic Injuries in Acute Cerebral Ischemic Stroke Mouse Model by Flexibly Regulating Mitochondria Dynamics
    Article Snippet: .. HT‐22 cells were prepared in 4 tubes for each FACS run: experimental group stained with both Calcein‐AM (2μM) and PI (1.5μM) (HY‐K1073, MCE, United States), single‐color controls for each fluorophore, and unstained cells for background determination. ..

    Recombinant:

    Article Title: ATP-Responsive Bimetallic Metal–Organic Frameworks Amplify Oxidative Stress in the Tumor Microenvironment for Synergistic Chemo-Immunotherapy
    Article Snippet: Mouse 4T1 cells were obtained from ATCC (Manassas, VA, USA; #CRL-2539). .. Recombinant murine GM-CSF (#HY-P7069), IL-4 (#HY-P7080), FAM (#HY-W021817), WST-1 (#HY-136976), Calcein-AM (#HY-D0041), and DCFH-DA (#HY-D0940) were purchased from MedChemExpress (Monmouth Junction, NJ, USA). .. The Annexin V-FITC/PI apoptosis detection kit (#C1062S) was purchased from Beyotime Biotechnology (Shanghai, China).

    Incubation:

    Article Title: Schisandrin B suppresses cholangiocarcinoma by targeting the ROS/p38 MAPK/NF‑κB axis
    Article Snippet: .. After treatment, cells were incubated with 2 μM calcein‐AM (CAS 148504‐34‐1; MedChemExpress) for 20 min at 37 ̊C, followed by a 5 min co‐incubation with 4 μM propidium iodide (PI; CAS 25535‐16‐4; MedChemExpress) at 37 ̊C. .. Fluorescence images were captured using a Cytation 3 Cell Imaging Multi‐Mode Reader (Agilent Technologies, Inc.) with excitation at 488 nm and emission recorded at 525 nm for calcein‐AM.

    Article Title: Schisandrin B suppresses cholangiocarcinoma by targeting the ROS/p38 MAPK/NF-κB axis
    Article Snippet: .. After treatment, cells were incubated with 2 μM calcein-AM (CAS 148504-34-1; MedChemExpress) for 20 min at 37°C, followed by a 5 min co-incubation with 4 μM propidium iodide (PI; CAS 25535-16-4; MedChemExpress) at 37°C. .. Fluorescence images were captured using a Cytation 3 Cell Imaging Multi-Mode Reader (Agilent Technologies, Inc.) with excitation at 488 nm and emission recorded at 525 nm for calcein-AM.

    Article Title: Role of iron and TfR1 in the application of high-dose ascorbate against pancreatic cancer
    Article Snippet: .. A total of 24 h after seeding, the cells were harvested by trypsinization, washed twice with PBS, and incubated after addition of PBS with 500 nM calcein AM (cat. no. C1359, MedChemExpress) for 15 min at 37°C in the dark. ..

    other:


    Article Title: Patient-derived kidney organoids recapitulate ADPKD and facilitate the identification of Rho pathway inhibitors as candidate therapeutics
    Article Snippet: After 6 h, kidney organoids were washed and live cell culture images were captured using EVOS M7000 (Invitrogen) (pulse).

    Cell Culture:

    Article Title: Patient-derived kidney organoids recapitulate ADPKD and facilitate the identification of Rho pathway inhibitors as candidate therapeutics.
    Article Snippet: Slides were washed three times with PBS (10 min each), followed by incubation with secondary antibodies (Abcam, 1:500) at room temperature for 1 h, then slides were washed in PBS and counterstained with DAPI (Abcam, 1:5000). .. The next day, organoids were treated with calcein-AM (1 μM, MCE) for 1h at 37◦C, the medium was then removed and the cells were washed twice with DMEM/F12 and live cell culture images were captured using EVOS M7000 (Invitrogen). .. Organoids were thoroughly removed from Matrigel or BME with Cell Recovery Solution (Corning) on ice before fixed.



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    ERO1L induces macrophage infiltration. A IHC staining showing F4/80 + , CD86 + , and CD206 + macrophage infiltration in the KC and KEC pancreas tissues harvested from the recovery phase. Scale bar: 50 μm. B In the recovery phase, F4/80 + , CD86 + , and CD206 + macrophage infiltration in the DMSO-treated or EN460-treated KC pancreas tissues were analyzed by IHC method. Scale bar: 50 μm. C Analyzing the correlation between ERO1L expression and various immune cells using the TCGA database. D Representative IHC staining images of ERO1L and CD68 in the pancreatic cancer specimens from Ren Ji cohort, scale bar: 100 μm. The correlation curve shows the correlation between ERO1L H-score and macrophage marker (CD68) in pancreatic cancer tissues. E Chemotaxis assay and statistical results of human macrophages <t>(THP-1)</t> treated with conditioned medium from sh-ERO1L and shCtrl pancreatic cancer cells (Capan-2 and MiaPaCa-2). **** P < 0.0001
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    ERO1L induces macrophage infiltration. A IHC staining showing F4/80 + , CD86 + , and CD206 + macrophage infiltration in the KC and KEC pancreas tissues harvested from the recovery phase. Scale bar: 50 μm. B In the recovery phase, F4/80 + , CD86 + , and CD206 + macrophage infiltration in the DMSO-treated or EN460-treated KC pancreas tissues were analyzed by IHC method. Scale bar: 50 μm. C Analyzing the correlation between ERO1L expression and various immune cells using the TCGA database. D Representative IHC staining images of ERO1L and CD68 in the pancreatic cancer specimens from Ren Ji cohort, scale bar: 100 μm. The correlation curve shows the correlation between ERO1L H-score and macrophage marker (CD68) in pancreatic cancer tissues. E Chemotaxis assay and statistical results of human macrophages <t>(THP-1)</t> treated with conditioned medium from sh-ERO1L and shCtrl pancreatic cancer cells (Capan-2 and MiaPaCa-2). **** P < 0.0001
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    Image Search Results


    ERO1L induces macrophage infiltration. A IHC staining showing F4/80 + , CD86 + , and CD206 + macrophage infiltration in the KC and KEC pancreas tissues harvested from the recovery phase. Scale bar: 50 μm. B In the recovery phase, F4/80 + , CD86 + , and CD206 + macrophage infiltration in the DMSO-treated or EN460-treated KC pancreas tissues were analyzed by IHC method. Scale bar: 50 μm. C Analyzing the correlation between ERO1L expression and various immune cells using the TCGA database. D Representative IHC staining images of ERO1L and CD68 in the pancreatic cancer specimens from Ren Ji cohort, scale bar: 100 μm. The correlation curve shows the correlation between ERO1L H-score and macrophage marker (CD68) in pancreatic cancer tissues. E Chemotaxis assay and statistical results of human macrophages (THP-1) treated with conditioned medium from sh-ERO1L and shCtrl pancreatic cancer cells (Capan-2 and MiaPaCa-2). **** P < 0.0001

    Journal: Cell & Bioscience

    Article Title: ERO1L induces macrophage infiltration to potentiate Kras G12D and inflammation-induced pancreatic tumorigenesis via oxidative protein folding of CCL2

    doi: 10.1186/s13578-026-01601-3

    Figure Lengend Snippet: ERO1L induces macrophage infiltration. A IHC staining showing F4/80 + , CD86 + , and CD206 + macrophage infiltration in the KC and KEC pancreas tissues harvested from the recovery phase. Scale bar: 50 μm. B In the recovery phase, F4/80 + , CD86 + , and CD206 + macrophage infiltration in the DMSO-treated or EN460-treated KC pancreas tissues were analyzed by IHC method. Scale bar: 50 μm. C Analyzing the correlation between ERO1L expression and various immune cells using the TCGA database. D Representative IHC staining images of ERO1L and CD68 in the pancreatic cancer specimens from Ren Ji cohort, scale bar: 100 μm. The correlation curve shows the correlation between ERO1L H-score and macrophage marker (CD68) in pancreatic cancer tissues. E Chemotaxis assay and statistical results of human macrophages (THP-1) treated with conditioned medium from sh-ERO1L and shCtrl pancreatic cancer cells (Capan-2 and MiaPaCa-2). **** P < 0.0001

    Article Snippet: Following a 12-h incubation of the THP-1 cells, the migrated cells were treated with 2 μM Calcein-AM (MCE, HY-D0041) for 15 min and subsequently observed under a fluorescence microscope.

    Techniques: Immunohistochemistry, Expressing, Marker, Chemotaxis Assay